NIIOS Publications

Scientific publications

2010
• Dapena I, Moutsouris K, Droutsas K, Ham L, van Dijk K, Melles GRJ. Standardized ‘no touch’ technique for Descemet’s membrane endothelial keratoplasty (DMEK): Controlled donor tissue implantation, orientation, unrolling, centering, appositioning and fixation. Arch of Ophthalmol. Accepted. 
• Ham L, Dapena L, Moutsouris K, Melles GRJ. Persistent corneal edema after descemetorhexis without corneal graft implantation in a case of Fuchs endothelial dystrophy. Cornea. Accepted.
• Dapena I, Ham L, Moutsouris K, Melles GRJ. Incidence of recipient Descemet membrane remnants at the donor-to-stromal interface after descemetorhexis in endothelial keratoplasty. Br J Ophthalmol. Accepted.
• Dapena I, Dapena L, Dirisamer M, Ham L, Melles GRJ. Visual acuity and endothelial cell density following Descemet membrane endothelial keratoplasty. Arch Soc Esp Oftalmol. Submitted.
• Villarrubia A, Fuentes-Páez G, Dapena I, Palacín E, Rodríguez Ausín P. Deep Anterior Lamellar Keratoplasty with Melles´ technique: Mid-term clinical outcome in relatively high risk population. Cornea. Submitted.
• Ham L, Dapena I, van der Wees J, Melles GRJ. Secondary DMEK for low visual outcome after DSEK: Donor posterior stroma may limit visual acuity in endothelial keratoplasty. Cornea. In press.
• Lie JT, Groeneveld-van Beek EA, Ham L, van der Wees J, Melles GRJ. More efficient use of donor corneal tissue with Descemet membrane endothelial keratoplasty (DMEK): two lamellar keratoplasty procedures with one donor cornea. Br J Ophthalmol 2010;94:1265-6.
• Moutsouris K, Ham L, Dapena I, van der Wees J, Melles GRJ. Radial graft contraction may relate to subnormal visual acuity in Descemet stripping (automated) endothelial keratoplasty. Br J Ophthalmol 2010;94:951-3.
• Droutsas K, Ham L, Dapena I, Geerling G, Oellerich S, Melles GRJ. Visus nach Descemet-Membran Endothelkeratoplastik (DMEK). Ergebnisse der ersten 100 Eingriffe bei Fuchs’scher Endotheldystrophie. Klin Monatsbl Augenheilkd 2010;227:467-77.
• Lie JT, Droutsas K, Ham L, Dapena I, Ververs B, Otten H, van der Wees J, Melles GRJ. Isolated Bowman layer transplantation to manage persistent subepithelial haze after excimer laser surface ablation. J Cataract Refract Surg 2010;36:1036-41.
• Ham L, Dapena I, van der Wees J, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK). 1-3 year follow-up. Am J Ophthalmol 2010;149:1016-7.
• Dapena I, Ham L, van Luijk C, van der Wees J, Melles GRJ. Back-up procedure for graft failure in Descemet Membrane Endothelial Keratoplasty (DMEK). Br J Ophthalmol 2010;94:241-4.
• Dapena I, Moutsouris M, Ham L, Melles GRJ. Graft detachment rate. Ophthalmology 2010;117:847-7e1.
• Balachandran C, Ham L, Dapena I, van der Wees J, Melles GRJ. Reply to Comment by FW Price et al on ‘Spontaneous corneal clearance despite graft detachment after Descemet membrane endothelial keratoplasty’. Am J Ophthalmol 2010;149:174-5.
• Balachandran C, Ham L, Dapena I, van der Wees J, Melles GRJ. Reply to Comment by Stewart et al on ‘Spontaneous corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty’. Am J Ophthalmol 2010;149:683-4.

2009
• Lie JT, Birbal R, Ham L, Dapena I, van der Wees J, Melles GRJ. Reply to Comment by Athanasiadis I et al on: Donor tissue preparation for Descemet membrane endothelial keratoplasty.  J Cataract Refract Surg 2009;35:408.
• Moutsouris K, Ham L, Dapena I, van der Wees J, Melles GRJ. Βραχυπροθεσμα αποτελεσματα της ενδοθηλιακης κερατοπλαστικης Δεσκεμετειου μεμβρανης (DMEK) για την αντιμεταπιση της ενδοθηλιακης δυστροφιας του Fuchs. Short term results of Descemet Membrane Endothelial Keratoplasty (DMEK) in the management of Fuchs endothelial dystrophy. Greek Ann Ophthalmol 2009;4:345-54.
• Nieuwendaal CP, van Velthoven MEJ, Biallosterski C, van der Meulen IJE, Lapid-Gortzak R, Melles GRJ, Verbraak FD. Thickness measurements of donor posterior discs after Descemet-stripping endothelial keratoplasty with anterior segment optical coherence tomography Cornea 2009;28:298-303.
• Ham L, Balachandran C, Verschoor AM, van der Wees J, Melles GRJ. Visual rehabilitation rate after isolated Descemet membrane transplantation: Descemet membrane endothelial keratoplasty. Arch Ophthalmol 2009;127:252-5.
• Ham L, van Luijk C, Dapena I, Wong TH, Birbal R, van der Wees J, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 1- to 2-year follow-up. Am J Ophthalmol 2009;148:521-7.
• Balachandran C, Ham L, Birbal R, Wong TH, van der Wees J, Melles GRJ. Simple technique for graft insertion in Descemet-stripping (automated) endothelial keratoplasty using a 30-gauge needle. J Cataract Refract Surg 2009;35:625-8.
• Ham L, Dapena I, van Luijk C, van der Wees J, Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy: review of the first 50 consecutive cases. Eye 2009;23:1990-8.
• Dapena I, Ham L, Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK - the thinner the better? Curr Opin Ophthalmol 2009;20:299-307.
• Balachandran C, Ham L, Verschoor CA, Ong TS, van der Wees J, Melles GRJ. Spontaneous Corneal Clearance Despite Graft Detachment in Descemet Membrane Endothelial Keratoplasty (DMEK). Am J Ophthalmol 2009;148:227-34.
• Dapena I, Ham L, Tabak S, Balachandran C, Melles GRJ. Phacoemulsification after Descemet membrane endothelial keratoplasty. J Cataract Refract Surg 2009;35:1314-5.
• Dapena I, Ham L, Lie JT, van der Wees J, Melles GRJ. Queratoplastia endotelial de membrana de Descemet (DMEK): Resultados a dos anos. Arch Soc Esp Oftalmol 2009;84:237-44.

2008
• Melles GRJ, Ong S, Ververs B, van der Wees J. Preliminary clinical results of Descemet membrane endothelial keratoplasty (DMEK). Am J Ophthalmol 2008;145:222-7.
• Ham L, van der Wees J, Melles GRJ. Causes of primary donor failure in Descemet membrane endothelial keratoplasty. Am J Ophthalmol 2008;145:639-44.
• Lie JT, Birbal R, Ham L, van der Wees J, Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). J Cataract Refract Surg 2008;34:1578-83.
• Lie JT, Lock FM, Mulder PGH, van der Wees J, Melles GRJ. Floating device for donor corneas in organ culture. Br J Ophthalmol 2008;92:1676-8.

2007
• Nieuwendaal CP, Lapid R, van der Meulen IJ, Melles GRJ. Posterior corneal graft detachment after organ culture (Letter). Cornea 2007;26:1024.
• van Dooren B, Mulder P, Nieuwendaal CP, Beekhuis WH, Melles GRJ. Endothelial cell density after posterior lamellar keratoplasty: five- to seven-year follow-up. Am J Ophthalmol 2007;144:471-3.

2006
• Nieuwendaal CP, Lapid R, van der Meulen IJ, Melles GRJ. Posterior lamellar keratoplasty using descemetorhexis and organ cultured donor corneal tissue (Melles technique). Cornea 2006;25:933-6.
• Melles GRJ, Ong S, Ververs B, Van der Wees J. Descemet membrane endothelial keratoplasty (DMEK). Cornea 2006;25:987-90.
• Melles GRJ. Posterior lamellar keratoplasty: DLEK to DSEK to DMEK (editorial). Cornea 2006;25:879-81.

2005
• Geerling G, Duncker GIW, Krumeich J, Melles GRJ. Lamellare keratoplasty. Ophthalmologe 2005;102:1140-8,1150-1. Review.

2004
• Dooren van BTH, Mulder P, Nieuwendaal CP, Beekhuis WH, Melles GRJ: Endothelial cell density after deep anterior lamellar keratoplasty (Melles technique). Am J Ophthal 2004;138:397-400.
• Dooren van BTH, Mulder P, Nieuwendaal CP, Beekhuis WH, Melles GRJ: Endothelial cell density after posterior lamellar keratoplasty (Melles technique); 3 years follow-up. Am J Ophthal 2004;138:211-7.
• Binder PS, Melles GRJ et al: Femtosecond laser in posterior lamellar keratoplasty. Review Refractive Surgery 2004.
• Aguilera F, Mohr A, Eckardt C, Wong D, Kusaka S, Joondeph BC, Feron EJ, Stalmans P, van Overdam K, Melles GRJ: Letter. Ophthalmology 2004.
• Melles GR, Wijdh RH, Nieuwendaal CP. A technique to excise the descemet membrane from a recipient cornea (descemetorhexis). Cornea. 2004;23:286-8.
• Melles GRJ. A disagreement. Ophthalmology 2004;111:193.
• Melles GRJ, Ververs B, JW van der Linden. Cosmetic extraocular implant. J Cataract Refract Surg 2004 (Letter). 2004;30:1595-6; discussion 1595.
• Melles GRJ. An Alternative to Penetrating Keratoplasty. Review of Ophthalmology en Espanol 2004.

2003
• Stalmans P, Feron EJ, Parys-van Ginderdeuren R, van Lommel A, Melles GRJ, Veckeneer M: Double vital staining using trypan blue and infracyanine green in macular pucker surgery. Br J Ophthalmol 2003;87:713-6.
• Melles GRJ, Kamminga N: Techniken der posterioren lamellären Keratoplastik über einen skleralen Zugang. Ophthalmologe 2003;100:689-95.
• Melles GRJ. Posterior lamellar keratoplasty, 6 different approaches. Klin Monatsbl. Augenheilkd 2003;220:S1-20.
• Melles GRJ. An Alternative to Penetrating Keratoplasty. Review of Ophthalmology 2003:67-72.
• Stalmans P et al: Trypan Blue not toxic for retinal pigment epithelium in vitro. Am J Ophthalmol 2003;135:234-6.

2002
• Feron EJ, Veckeneer M, Parys R, Van Lommel A, Melles GRJ, Stalmans P: Trypan blue staining of epiretinal membranes in proliferative vitreoretinopathy. Arch Ophthalmol 2002:120:141-4.
• Melles GRJ, Lander F, Nieuwendaal CP: Sutureless posterior lamellar keratoplasty. Cornea 2002;21:325-7.
• Melles GRJ: Editorial on posterior lamellar keratoplasty. Arch Soc Esp Oftalmol 2002;77:175-6.
• de Waard PTW, Budo C, Melles GRJ: Trypan blue capsular staining to visualize a ‘lost’ capsulorhexis. Am J Ophthalmol 2002;134:271-2.
• Melles GRJ, Lander F, Rietveld FJR: Transplantation of Descemet’s membrane carrying viable endothelium through a small scleral incision. Cornea 2002;21:415-8.
• van Nouhuijs H, van den Berg W, Melles GRJ: Trypan blue stained visco elastic for ophthalmic surgery. J Cataract Refract Surg 2002;28:574-5.
• van Dooren BTH, de Waard PTW, van Nouhuijs HM, Beekhuis WH, Melles GRJ: Corneal endothelial cell density after trypan blue capsule staining in cataract surgery. J Cataract Refract Surg 2002;28:574-5.
• Odenthal MT, Eggink CA, Melles GRJ, Pameyer JH, Geerards AJ, Beekhuis WH: Clinical and theoretical results of intraocular lens power calculation for cataract surgery after photorefractive keratectomy for myopia. Arch Ophthalmol. 2002;120:431-8.
• Melles GRJ, Lander F, Rietveld FJR: Comment on Transplantation of Descemet’s membrane carrying viable endothelium through a small scleral incision (Letter). Cornea 2002;21:840.
• Aguilera F, Mohr A, Eckardt C, Wong D, Kusaka S, Joondeph BC, Feron EJ, Stalmans P, van Overdam K, Melles GRJ: Trypan blue staining in vitreoretinal surgery. Ophthalmology 2003;110:2409-12.
• Alio JL, Shah S, Barraquer C, Bilgihan K, Anwar M, Melles GRJ: New techniques in lamellar keratoplasty. Current Opinion in Ophthalmology 2002;13:224-9.

2001
• Caroline PJ, Melles GRJ: Two cases of bilateral, stromal ring opacity of the cornea. Cornea, 2001;20:237-8.
• Paridaens D, Beekhuis WH, van den Bosch W, Remeyer L, Melles GRJ: Amniotic membrane transplantation in the management of conjunctival malignant melanoma and primary acquired melanosis with atypia. Br J Ophthalmol 2001;85:658-61.

2000
• Melles GRJ, Remeijer L, Geerards AJM, Beekhuis WH: A quick surgical technique for deep lamellar keratoplasty using visco-dissection. Cornea 2000;19:427-32.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Preliminary clinical results of posterior lamellar keratoplasty through a sclerocorneal pocket incision. Ophthalmology 2000;107:1850-7.
• Melles GRJ: Lamellar Keratoplasty. Moorfields On-line Textbook on Ophthalmology <www.eyetext.net>
• Melles GRJ: Mature Cataract Surgery. Moorfields On-line Textbook on Ophthalmology www.eyetext.net

1999
• Eggink FAGJ, Melles GRJ, Rietveld FJR, Eggink CA, Beekhuis WH, Binder PS: Topography following keratoplasty in cats is not representative for that in humans. J Refract Surg 1999;15:74-5.
• Melles GRJ, Rietveld FJR, Beekhuis WH, Binder PS: A technique to visualize corneal incision and lamellar dissection depth during surgery. Cornea 1999;18:80-6.
• Melles GRJ, Lander F, Beekhuis WH, Remeijer L, Binder PS: Posterior lamellar keratoplasty for a case of pseudophakic bullous keratopathy. Am J Ophthalmol 1999;127:340-1.
• Melles GRJ, de Waard PWT, Pameijer JH, Beekhuis WH: Trypan blue capsule staining to visualize the capsulorhexis in cataract surgery. J Cataract Refract Surg 1999;25:7-9.
• Melles GRJ, Lander F, Rietveld FJR, Remeijer L, Beekhuis WH, Binder PS: A new surgical technique for deep stromal, anterior lamellar keratoplasty. Br J Ophthalmol 1999;83:327-33.
• Melles GRJ, Remeijer L, Geerards A, Beekhuis WH: The future of lamellar keratoplasty. Curr Opin Ophthalmol 1999;10:253-9.
• Melles GRJ, de Waard PTW, Pameijer JH, Beekhuis WH: Trypanblau Färbung der Linsenkapsel zur Visualisierung der Kapsulorhexis bei Maturkatataktchirurgie. Fortschr Ophthalmol. 1999:215:342-4.

1998
• Melles GRJ, Rietveld FJR, Beekhuis WH, Binder PS: Depth predictability of stromal pockets in the posterior cornea. Cornea 1998;17:174-9.
• Melles GRJ, de Séra P, Eggink CA, Cruysberg JRM, Binder PS: Bilateral, anterior stromal ring opacity of the cornea. Br J Ophthalmol 1998;82:522-5.
• Melles GRJ, Eggink FAGJ, Lander F, Pels E, Rietveld FJR, Beekhuis WH, Binder PS: A surgical technique for posterior lamellar keratoplasty. Cornea 1998;17:618-26.

Book chapters

• Dapena I, Moutsouris K, Droutsas K, Ham L, van Dijk K, Melles GRJ. Queratoplastia endotelial de membrana de Descemet (DMEK): Técnica quirúrgica. In Villarrubia A (ed.): Trasplante del endotelio corneal. 2010:173-83.
• Dapena I, Ham L, Dirisamer M, Melles GRJ. DMEK: resultados y comparación con DSEK/DSAEK. In Villarrubia A (ed.): Trasplante del endotelio corneal. 2010:185-95.
• Melles GRJ. Deep anterior lamellar keratoplasty. Essentials in Ophthalmology: Cornea and External Eye Disease. Springer Verlag, 2006:65-71.
• Melles GRJ. Deep anterior lamellar keratoplasty. In: Vajpayee RB (ed): Corneal Transplantation. Lippincott, 2002:167-82.
• Melles GRJ, Alio JL. Deep anterior lamellar keratoplasty to correct irregular astigmatism after refractive surgery. In: Alio JL (ed): Refractive Surgery. Highlights of Ophthalmology International, 2002:183-4.

Upcoming presentations

• Dapena I. Descemetorhexis under air may reduce the risk of recipient descemet membrane remnants at the donor to stromal interface after endothelial keratoplasty. ESCRS, Paris, France, September 2010.
• van Dijk K. Deep lamellar endothelial keratoplasty (DLEK) 9-12 year follow up of first patient cohort worldwide. ESCRS, Paris, France, September 2010.
• Melles GRJ. Endothelial cell density after descemet membrane endothelial keratoplasty (DMEK) 3 year follow up. ESCRS, Paris, France, September 2010.
• Melles GRJ. Visual rehabilitation following descemet membrane endothelial keratoplasty (DMEK). ESCRS, Paris, France, September 2010.
• Dapena I. Learning curve in descemet membrane endothelial keratoplasty (DMEK). First series of 135 consecutive cases. ESCRS, Paris, France, September 2010.
• Melles GRJ. Restoration of a functional corneal endothelium, present and future therapeutic possibilities. Danish Ophthalmological Society, Copenhagen, Denmark, September 2010.
• Dapena I, Moutsouris K, Droutsas K, Ham L, van Dijk K, Melles GRJ. Queratoplastia endothelial de membrane de Descemet (DMEK): Técnica quirúrgica. Ponencia de la Sociedad Espanola de Oftalologia, Madrid, Spain, September 2010.
• Dapena I, Ham L, Dirisamer M, Melles GRJ. DMEK: resultados y comparación con DSEK/DSAEK. Ponencia de la Sociedad Espanola de Oftalmologia, Madrid, Spain, September 2010.
• Melles GRJ. DSEK, DSAEK or DMEK – The thinner the better? Linzer Ophthalmologische Gespräche, Linz, Austria, October 2010.
• Melles GRJ et al. Interaktive Sprechstunde mit Paneldiskussion. Linzer Ophthalmologische Gespräche, Linz, Austria, October 2010.
• Melles GRJ et al. Innovationen in der Ophthalmochirurgie: Was habe ich in den letzen beiden Jahren an meiner Operationstechnik geandert? Linzer Ophthalmologische Gespräche, Linz, Austria, October 2010.
• Dirisamer M. Nicht perforierende Hornhauttransplantation. Linzer Ophthalmologische Gespräche, Linz, Austria, October 2010.
• Melles GRJ. Endothelzelldichte nach ‘Descemet-Membran Endothelkeratoplastik (DMEK): Ergebnisse nach 3 Jahren. DOC, Hamburg, Germany, October 2010.
• Melles GRJ. Visus nach Descemet Membran Endothelkeratoplastik (DMEK). DOC, Hamburg, Germany, October 2010.
• Melles GRJ. Title pending. Keratoplasty 2010. Bilbao, Spain, November 2010.  

Presentations

2010
• Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK: the thinner, the better? Cairo Middle East Anterior Segment and Refractive Surgery Symposium, Cairo, Egypt, January 2010 (invitation).
• Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). Cairo Middle East Anterior Segment and Refractive Surgery Symposium, Cairo, Egypt, January 2010 (invitation).
• Melles GRJ. DMEK surgical techniques (video). Cairo Middle East Anterior Segment and Refractive Surgery Symposium, Cairo, Egypt, January 2010 (invitation).
• van der Wees J, Ham L, Dapena I, Moutsouris K, Droutsas K, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 3 years follow-up. European Eye Bank Association (EEBA), Sitges, Spain, January 2010.
• Ham L, Dapena I, Moutsouris K, Droutsas K, Melles GRJ. Visual rehabilitation following Descemet membrane endothelial keratoplasty (DMEK). European Eye Bank Association (EEBA), Sitges, Spain, January 2010.
• Lie JL, Groeneveld EA, van der Wees J, Melles GRJ. More efficient use of donor corneas by using Descemet Membrane Endothelial Keratoplasty (DMEK). European Eye Bank Association (EEBA), Sitges, Spain, January 2010.
• Μουτσουρης Κ, Ham L. Διαυγαση κερατοειδους παρα την αποκολληση του δεσκεμετονδοθηλιακου μοσχευματος μετα απο DMEK. HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Μουτσουρης Κ. Πληρης οπτικη αποκατασταση μετα απο αντικατασταση ενδοθηλιακου μοσχευματος: Δευτερογενης DMEK μετα απο DSEK. Complete visual recovery after endothelial keratoplasty graft exchange: DMEK after DSEK. HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Dapena I, Droutsas K, Ham L, Moutsouris K, van der Wees J, Melles GRJ. Back-up procedure for graft failure in Descemet membrane endothelial keratoplasty (DMEK). HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Ham L, Moutsouris K, Droutsas K, Dapena I, Balachandran C, van der Wees J, Melles GRJ. Corneal Clearance despite Graft Detachment in Descemet Membrane Endothelial Keratoplasty. HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Droutsas K, Moutsouris K, Ham L, Dapena I, van der Wees J, Melles GRJ. Visual rehabilitation after Descemet membrane endothelial keratoplasty (DMEK) - Results of the first 100 cases. HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Miltsakakis D, Droutsas K. Instruction course. Endothelial keratoplasty: DSAEΚ and DMEK. HSIOIRS CONGRESS, Athens, Greece, February 2010.
• Melles GRJ. DSEK/DSAEK or DMEK: the thinner, the better? Fuchs Corneal Dystrophy Symposium. Baltimore, MD, USA, February 2010.
• Dapena I. Standardized ‘no touch’ technique for Descemet membrane endothelial keratoplasty (DMEK). Fuchs Corneal Dystrophy Symposium. Baltimore, MD, USA, February 2010.
• Droutsas K. Complications of DMEK. Fuchs Corneal Dystrophy Symposium. Baltimore, MD, USA, February 2010.
• Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK: the thinner, the better? Barcelona, Spain, March 2010.
• van der Wees J, Ham L, Dapena I, Droutsas K, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 3 years follow-up. NOG, Maastricht, Netherlands, March 2010.
• Ham L, Dapena I, Droutsas K, Melles GRJ. Visual rehabilitation and detachment rate following Descemet membrane endothelial keratoplasty (DMEK): first 135 eyes. NOG, Maastricht, Netherlands, March 2010.
• Dapena I, Balachandran C, Ham L, Droutsas K, Melles GRJ. Spontaneous corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty (DMEK). NOG, Maastricht, Netherlands, March 2010.
• Melles GRJ, Dapena I, Ham L, Droutsas K. Endothelial keratoplasty: DSEK/DSAEK or DMEK - the thinner the better? NOG, Maastricht, Netherlands, March 2010.
• Lie JT, Groeneveld EA, van der Wees J, Melles GRJ. More efficient use of donor corneas with Descemet membrane endothelial keratoplasty. NOG, Maastricht, Netherlands, March 2010.
• Melles GRJ. Is DMEK the preferred method of PLK? How can corneal surgeons begin doing this? WOC, Berlin, Germany, June 2010.
• Melles GRJ. DLEK, DSAEK, DMEK, the thinner the better. WOC, Berlin, Germany, June 2010.
• Lie J. Is DMEK the preferred method of endothelial keratoplasty? Singapore National Eye Centre, Singapore, Singapore, July 2010.

2009
• Dapena I, Ham L, van Luijk CM, van der Wees, J, Melles GRJ. Visual rehabilitation after Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy. European Eye Bank Association (EEBA), Amsterdam, Netherlands, January 2009.
• Van Luijk CM, Birbal R, Lie JT, van der Wees J, Melles GRJ. Assessment of corneal endothelial cell damage induced by a complete air-fill of the anterior chamber of the eye. EEBA, Amsterdam, Netherlands, January 2009.
• Ham L, Dapena I, van der Wees J, Melles GRJ. Visual rehabilitation rate after isolated Descemet membrane transplantation: Descemet membrane endothelial keratoplasty (DMEK). Hallesches Symposium, Halle, Germany, January 2009.
• Dapena I, Ham L, van Luijk CM, Lie JT, van der Wees J, Melles GRJ. Spontaneous corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty (DMEK). Winter ESCRS, Rome, Italy, February 2009.
• Dapena I, Ham L, van Luijk CM, Verschoor C, van der Wees J, Melles GRJ. Descemet stripping endothelial keratoplasty (DSEK) after Descemet membrane endothelial keratoplasty (DMEK). Winter ESCRS, Rome, Italy, February 2009.
• Dapena I, Ham L, van Luijk CM, Lie JT, Birbal R, van der Wees J, Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). Winter ESCRS, Rome, Italy, February 2009.
• Ham L, Balachandran C, Dapena I, Verschoor C, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 2 years follow-up. Winter ESCRS, Rome, Italy, February 2009. 
• Ham L, Balachandran C, Dapena I, Verschoor C, Melles GRJ. Visual rehabilitation following Descemet membrane endothelial keratoplasty (DMEK). Winter ESCRS, Rome, Italy, February 2009. 
• Melles GRJ. Crosslinking and other treatment options for keratoconus. Optometristen Vereniging Nederland, Ermelo, Netherlands, February 2009 (invitation). 
• Van Luijk CM, Birbal R, Lie JT, Ham L, van der Wees J, Melles GRJ. Corneal endothelial cell damage by complete air-fill of the anterior chamber. NOG, Groningen, Netherlands, March 2009.
• Lie JT, Birbal R, Ham L, van der Wees J, Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). NOG, Groningen, Netherlands, March 2009.
• Dapena I, Van Luijk CM, Ham L, van der Wees J, Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy. Review of the first 50 consecutive cases. NOG, Groningen, Netherlands, March 2009.
• Van Luijk CM, Ham L, van der Wees J, Balachandran C, Dapena I, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 2 years follow-up. NOG, Groningen, Netherlands, March 2009.
• Van Luijk CM, Birbal R, Lie JT, Ham L, van der Wees J, Melles GRJ. Corneal endothelial cell damage by complete air-fill of the anterior chamber. NOG, Groningen, Netherlands, March 2009.
• Melles GRJ. Refractive surgical corneal complications. NGRC, Amsterdam, Netherlands, May 2009.
• Lie JT, Birbal R, Ham L, van der Wees J, Melles GRJ. Donor tissue preparation for Descemet Membrane Endothelial keratoplasty (DMEK). Reunion de la federation de banques de tissus oculaires, Paris, France, May 2009.
• Dapena I. Preparación del tejido donante para la queratoplastia endotelial de membrana Descemet (DMEK). 24 Congreso SECOIR, San Sebastián, Spain, May 2009.
• Dapena I. Recuperación espontánea de la transparencia corneal a pesar del desprendimiento del injerto en la queratoplastia endotelial de membrana Descemet (DMEK). 24 Congreso SECOIR, San Sebastián, Spain, May 2009.
• Dapena I. Densidad celular endotelial tras la queratoplastia endotelial de membrana de Descemet (DMEK): resultados a 1 año. 24 Congreso SECOIR, San Sebastián, Spain, May 2009.
• Dapena I. Rehabilitación visual después de la queratoplastia endotelial de membrana de Descemet (DMEK). 24 Congreso SECOIR, San Sebastián, Spain, May 2009.
• Μουτσουρης Κ, Dapena I, Ham L, Van Luijk CM, Van der Wees J, Melles GRJ. Οπτικη αποκατασταση μετα απο μετα απο επιλεκτικη μεταμοσχευση ενδοθηλιου και Δεσκεμετειου μεμβρανης (DMEK). 42nd Panhellenic Ophthalmological Congress, Crete, Greece, May 2009.
• Μουτσουρης Κ, Dapena I, Ham L, Lie JT, Van der Wees J, Melles GRJ. Πυκνοτητα ενδοθηλιακων κυτταρων μετα απο επιλεκτικη μεταμοσχευση ενδοθηλιου και Δεσκεμετειου μεμβρανης (DMEK): follow up 2 ετων. 42nd Panhellenic Ophthalmological Congress, Crete, Greece, May 2009.
• Dapena I, Ham L, van Luijk CM, Balachandran C, Verschoor C, Melles GRJ. Visual rehabilitation following Descemet membrane endothelial keratoplasty (DMEK). SOE, Amsterdam, Netherlands, June 2009.
• Melles GRJ. DSEK/DSAEK or DMEK: the thinner the better?. SOE, Amsterdam, Netherlands, June 2009.
• Moutsouris K. Descemet membrane endothelial keratoplasty (DMEK): clinical outcome of first one hundred patients. SOE, Amsterdam, Netherlands, June 2009.
• Melles GRJ. Lamellar Keratoplasty. DOC, Nürnberg, Germany, June 2009.
• Melles GRJ. Anterior Lamellar Keratoplasty. DOC, Nürnberg, Germany, June 2009.
• Melles GRJ. Posterior Lamellar Keratoplasty. DOC, Nürnberg, Germany, June 2009.
• Melles GRJ. DSAEK and DMEK - Current positions in endothelial lamellar keratoplasty. DOC, Nürnberg, Germany, June 2009.
• Lie JT, Ham L, Van der Wees J, Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK): What do we need? DOG, Leipzig, Germany, September 2009.
• Dapena I, Balachandran C, Ham L, Moutsouris K, Melles GRJ. Spontaneous corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty (DMEK). ESCRS, Barcelona, Spain, September 2009.
• Dapena I, Ham L, Moutsouris K, van der Wees J, Melles GRJ. Back-up procedure for graft failure in Descemet Membrane Endothelial Keratoplasty (DMEK). ESCRS, Barcelona, Spain, September 2009.
• Dapena I, Moutsouris K, Ham L, van der Wees J, Melles GRJ. Incidence or retained recipient Descemet's membrane after descemetorhexis in endothelial keratoplasty. ESCRS, Barcelona, Spain, September 2009.
• Moutsouris K, Dapena I, Ham L, van der Wees J, Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK) after Descemet Stripping Endothelial Keratoplasty (DSEK). ESCRS, Barcelona, Spain, September 2009.
• Moutsouris K, Lie JT, Ham L, van der Wees J, Melles GRJ. Donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK): what do we need? ESCRS, Barcelona, Spain, September 2009.
• Ham L, Dapena I, Moutsouris K, Melles GRJ. Endothelial cell density after Descemet membrane endothelial keratoplasty (DMEK): 1-2 years follow-up. ESCRS, Barcelona, Spain, September 2009.
• Ham L, Dapena I, Moutsouris K, Melles GRJ. Visual rehabilitation following Descemet membrane endothelial keratoplasty (DMEK). ESCRS, Barcelona, Spain, September 2009.
• Dapena I. Preparación del tejido donante para la queratoplastia endotelial de membrana Descemet (DMEK). Congreso de la Sociedad Española de Oftalmología, Santander, Spain, September 2009.
• Dapena I. Queratoplastia endotelial de membrana de Descemet (DMEK: Descemet membrane endothelial keratoplasty). Congreso de la Sociedad Española de Oftalmología, Santander, Spain, September 2009.
• Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK - The thinner the better? HOS, Sao Paolo, Brazil, November 2009 (invitation).
• Melles GRJ. The future of endothelial keratoplasty. HOS, Sao Paolo, Brazil, November 2009 (invitation).
• Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK: the thinner, the better? Rohovkový Kongres, Corneal Day. Prague, Czech Republic, December 2009 (invitation).

2008
• Lie JT, van der Wees J, Melles GRJ. Manually pre-dissected donor corneas for Descemet stripping endothelial keratoplasty (DSEK). EEBA, Muenich, Germany, January 2008.
• Ham L, Lie JT, Ong TS, van der Wees J, Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). EEBA, Muenich, Germany, January 2008.
• van der Wees J, Ham L, Melles GRJ. Causes of primary donor failure in Descemet membrane endothelial keratoplasty (DMEK). EEBA, Muenich, Germany, January 2008.
• Ham L, Lie JT, Ong TS, van der Wees J, Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). ESCRS Winter Refractive meeting, Barcelona, Spain, February 2008.
• Melles GRJ. Title pending. 22nd International Congress Hellenic Society Intraocular Implant and Refractive Surgery. Athens, Greece, February 2008 (invitation).
• Melles GRJ. Update on endothelial keratoplasty. Italian Society Of Corneal Transplantation (SITRAC), Milan, Italy, February 2008 (invitation).
• Melles GRJ. 1-2 year clinical results of Descemet membrane endothelial keratoplasty (DMEK). NOG, Maastricht, The Netherlands, April 2008.
• van der Wees J. Primary donor failure after Descemet membrane endothelial keratoplasty (DMEK). NOG, Maastricht, The Netherlands, April 2008.
• Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK). ASCRS, Chicago, US, April 2008 (invitation).
• Melles GRJ. Endothelial keratoplasty. Bowman meeting, Liverpool, UK, April 2008 (invitation).
• Balachandran C, Melles GRJ. Endothelial keratoplasty. Vienna Ophthalmologic Society (WOG), Vienna, Austria, May 2008 (invitation).
• Balachandran C, Melles GRJ. Endothelial keratoplasty. Portuguese Refractive Congress, Albufeira, Portugal, May 2008 (invitation).
• Melles GRJ. Cataract and Refractive meeting. Prague, Czech Republic, May 2008 (invitation).
• Melles GRJ. Wet-lab Lamellierende Keratoplastik-Techniken W-HH1a & W-HH1b. DOC, Nurnberg, Germany, June 2008 (invitation).
• Ham L, Balachandran C, Verschoor CA, Melles GRJ. Visual rehabilitation following Descemet membrane endothelial keratoplasty (DMEK). DOC, Nurnberg, Germany, June 2008.
• Ham L, Balachandran C, Verschoor CA, Melles GRJ. Endothelial cell density in Descemet membrane endothelial keratoplasty (DMEK): 12 months follow up. DOC, Nürnberg, Germany, June 2008.
• Balachandran C, Ham L, Verschoor CA, Melles GRJ. Comparison of Optical Tomography (OCT) and Scheimpflug Pentacam imaging of Descemet stripping endothelial keratoplasty (DSEK) versus Descemet membrane endothelial keratoplasty (DMEK). DOC, Nürnberg, Germany, June 2008.
• Balachandran C, Ham L, Verschoor CA, Melles GRJ. Refractive outcome after Descemet membrane endothelial keratoplasty (DMEK). DOC, Nürnberg, Germany, June 2008.
• Balachandran C, Ham L, Verschoor CA, Melles GRJ. Causes of primary donor failure in Descemet membrane endothelial keratoplasty (DMEK). DOC, Nürnberg, Germany, June 2008.
• Melles GRJ. Endothelial keratoplasty. Wilmer Eye Institute - Johns Hopkins University, Baltimore, US, September 2008 (invitation).
• Melles GRJ. Endothelial keratoplasty. Cambridge Ophthalmological symposium, Cambridge, UK, September 2008 (invitation).
• Dapena I, Ham L, van Luijk CM, van der Wees, J, Melles GRJ. Visual rehabilitation rate after Descemet membrane Endothelial keratoplasty. ESCRS, Berlin, Germany, September 2008.
• Dapena I, Ham L, van Luijk CM, van der Wees, J, Melles GRJ. Descemet Membran Endothelkeratoplastik. DOG, Berlin, Germany, September 2008 (invitation).
• Dapena I, Ham L, van Luijk CM, van der Wees, J, Melles GRJ. Descemet Membran Endothelkeratoplastik. München, Germany, October 2008 (invitation).
• Dapena I, Ham L, van Luijk CM, van der Wees, J, Melles GRJ. Visual rehabilitation after Descemet membrane endothelial keratoplasty (DMEK). AAO, Atlanta, US, November 2008.
• Dapena I, Ham L, Lie JT, Birbal RS, van der Wees, J, Melles GRJ. Donor tissue preparation in Descemet membrane endothelial keratoplasty (DMEK). AAO, Atlanta, US, November 2008.
• Van Luijk CM, Birbal R, Lie JT, van der Wees J, Melles GRJ. Assessment of corneal endothelial cell damage induced by a complete air-fill of the anterior chamber of the eye. ARVO-NED, Leiden, The Netherlands, November 2008.
• Melles GRJ. 17th Iranian Ophthalmology Congress, Tehran, Iran, November 2008 (invitation).
• Melles GRJ. Lamellar keratoplasty. Amman Eye Hospital Meeting, Jordan, 2008 (invitation).

2007
• Melles GRJ, Ververs B: Wetlab and live surgery lamellar keratoplasty - Descemet stripping endothelial keratoplasty (DSEK). Hallesches Symposium. Halle/Saale, Germany, January 2007 (invitation).
• Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK). Hallesches Symposium. Halle/Saale, Germany, January 2007 (invitation).
• Wees J, Ong TS, Ververs B, Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK). European Eyebank Association, Bratislava, January 2007.
• Lie JT, Wees J, Lock FM, Melles GRJ. Cornea claw - Corneal suspension device in organ culture. European Eyebank Association, Bratislava, January 2007.
• Melles GRJ. Lamellaere Keratoplastik. Symposium Universitätsklinikum Essen, Germany, March 2007 (invitation).
• Melles GRJ. Descemet membrane endothelial keratoplasty (DMEK). NOG. Rotterdam, Netherlands, March 2007.
• Melles GRJ. Moderne Techniken fuer Lamellaere Keratoplastik. Instruction course HH1, DOC. Nürnberg, Germany, May 2007 (invitation).
• Melles GRJ. Wet-lab Lamellierende Keratoplastik-Techniken H1a & H1b. DOC, Nürnberg, Germany, May 2007 (invitation).
• van Velthoven MEJ, Biallosterski C, Verbraak FD, van der Meulen IJE, Melles GRJ, Nieuwendaal CP. Thickness measurement of the posterior lamella after posterior lamellar keratoplasty, with the anterior segment OCT. ARVO, Fort Lauderdale, USA, May 2007.
• Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). Oxford Ophthalmological Congress, London, UK, July 2007 (invitation).
• Melles GRJ. Deep anterior lamellar keratoplasty (DALK). Oxford Ophthalmological Congress, London, UK, July 2007 (invitation).
• Melles GRJ. VisionBlue - Erweitere Applikationsmoglichkeiten. Rayner-Forum, Bamberg, Germany, July 2007 (invitation).
• Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). ESCRS, Stockholm, Sweden, September 2007.
• Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). Cornea Clinical Conference United, Iowa-City, US, September 2007(invitation).
• Melles GRJ. Causes of failure in DSEK and DMEK. Cornea Clinical Conference United Iowa-City, US, September 2007 (invitation).
• Melles GRJ. Deep anterior lamellar keratoplasty. Review & future perspectives. Cornea Clinical Conference United, Iowa-City, US, September 2007 (invitation).
• Melles GRJ. Erste Ergebnisse der Descemet-Membran Endothel-Keratoplastik (DMEK). DOG, Berlin, Germany, September 2007 (invitation).
• Lie JT. Cornea’s: Van donor tot patient. Minisymposium corneadonatie en transplantatie IJsselland Ziekenhuis, Capelle a/d IJssel, The Netherlands, October 2007 (invitation).
• Melles GRJ. DLEK to DSEK to DMEK. EVER, Portoroz, Slovenia, October 2007 (invitation).
• Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). AAO, New Orleans, November 2007.
• Melles GRJ. Preliminary results of Descemet membrane endothelial keratoplasty (DMEK). BSCRS, Brussels, Belgium, November 2007 (invitation).
• Balachandran C, Dapena I, Ham L, van Luijk CM, van der Wees J, Melles GRJ. Corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty (DMEK). AAO, New Orleans, November 2007.

2006
• Melles GRJ: Update in lamellar keratoplasty techniques. NIIOS Corneal transplant meeting. Rotterdam, Netherlands, February 2006.
• van Dooren B, Mulder P, Beekhuis H, Melles G. Endothelial cell density (ECD) after posterior lamellar keratoplasty (PLK): 5-7 year follow-up. ARVO, Fort Lauderdale, USA, 2006.
• Melles GRJ. Lamellar corneal surgery for complications after refractive surgery. NGRC. Rotterdam, Netherlands, March 2006 (invitation).
• Melles GRJ. Complications in DSEK. Instruction course HH1, DOC. Nürnberg, Germany, May 2006 (invitation).
• Melles GRJ. Posterior lamellar keratoplasty / Descemet stripping endothelial keratoplasty. APAO, Singapore, June 2006 (invitation).
• Tan D, Melles GRJ. Techniques in optical, therapeutic and tectonic lamellar keratoplasty (DSEK). Instruction course 21, APAO, Singapore, June 2006 (invitation).
• Lie J. Stamceltransplantatie in de Oogheelkunde. EUR, Rotterdam, Netherlands, June 2006.
• Melles GRJ. Posterior lamellar keratoplasty / Descemet stripping endothelial keratoplasty. Update on corneal transplantation and cataract surgery. Tehran, Iran, June 2006 (invitation).
• Melles GRJ. Wet-lab Keratoplastik-Fortgeschrittenen-Kurs “Lamellaierende Keratoplastik-Techniken”. DOG, Berlin, Germany, September 2006 (invitation).
• Melles GRJ: Update in lamellar keratoplasty techniques. NIIOS Corneal transplant meeting, Rotterdam, October 2006.
• Melles GRJ, Ong TS, Ververs B, van der Wees J. Descemet membrane endothelial keratoplasty (DMEK). Castroviejo Cornea Society Meeting, Las Vegas, November 2006.
• Melles GRJ, Giebel A, Price FW, Rootman DS, Hamill B, Tappin M: Posterior lamellar keratoplasty without corneal incisions or sutures and Descemet membrane transplantation. AAO, Instruction course 369,  Las Vegas, November 2006.
• Update on techniques for posterior lamellar keratoplasty: DLEK to DSEK to DMEK. Iranian Congress of Ophthalmology, Tehran, Iran, November 2006 (invitation).
• Melles GRJ. Posterior lamellar keratoplasty. Cornea: Opinioni a Confronto, Florence, Italy, November 2006 (invitation).

2005
• Melles GRJ: Update in lamellar keratoplasty techniques. NIIOS Corneal transplant meeting, Rotterdam, Netherlands, September 2005.
• Melles GRJ, Giebel A, Price FW, Rootman DS, Hamill B: Posterior lamellar keratoplasty without corneal incisions or sutures and Descemet membrane transplantation. AAO, Instruction course 539,  Chicago, USA, October 2005.

2004
• Melles GRJ. Posterior lamellar keratoplasty in 2004. Techniques, indications and results. SAFIR, Paris, France, May 2004.
• Melles GRJ. Changing the endothelium. European Society of Corneal and Refractive Surgeons. Paris, France, September 2004.
• Melles GRJ. Panalist Free paper session: Cataract surgery paediatric. European Society of Corneal and Refractive Surgeons. Paris, France, September 2004 (invitation).
• Melles GRJ, Rootman DS, Price FW, Giebel A: Posterior lamellar keratoplasty without corneal incisions or sutures and Descemet membrane transplantation. AAO, Instruction course 459, New Orleans, USA, October 2005.

2003
• Melles GRJ: Posterior lamellar keratoplasty. 26th Congress of Japanese Ophthalmic Surgery, Tokyo, Japan, January 2003. (Invitation).
• Melles GRJ: Lamellar keratoplasty. DOC, Nürnberg, Germany, June 2003 (invitation).
• Melles GRJ: Quaratoplastia Laminar Profunda. Instituto Barraquer de America, Bogota, Colombia, August 2003 (invitation).
• Melles GRJ: Posterior lamellar keratoplasty. Fondation Rothschild, Paris, France, October 2003 (invitation).
• Melles GRJ, van Dooren BTH, Nieuwendaal CP, et al. Posterior lamellar keratoplasty, 3-5 years postoperative results. Eye Bank Association of America. Anaheim, USA, November 2003.
• Melles GRJ: Toward the transplantation of Descemet’s membrane: Descemetorhexis. Eye Bank Association of America. Anaheim, USA, November 2003.
• Serdarevic ON, Baudouin C, Culbertson WW, Jackson SB, Maldonado-Bas AR, Melles GRJ, Mills RP, Kaz Soong, Stark WJ, van Meter WS. Current concepts and new techniques in corneal transplantation. Instruction course 172, AAO, Anaheim, USA, November 2003.
• Aguilera F, Melles GRJ, Oncel M, Joondeph B. Trypan blue staining in vitreoretinal surgery. Instruction course 570, AAO, Anaheim, USA, November 2003.
• Melles GRJ. Posterior lamellar keratoplasty. MCLOSA, London, UK, November 2003 (invitation).
• Melles GRJ. Posterior lamellar keratoplasty, 6 different approaches. BBAG, Berlin, Germany, December 2003 (invitation).

2002
• Melles GRJ: Posterior lamellar keratoplasty. Cornea meeting, Paris, France, January 2002. (Invitation).
• Geerling G, Melles GRJ, Seitz B: Spezielle Keratoplastik-Techniken. AAD, Düsseldorf, Germany, March 2002. (Invitation).
• Melles GRJ: Lamellar keratoplasty. Sunderland Eye Infirmary, Sunderland, UK, March 2002. (Invitation).
• Melles GRJ: Transplantation of Descemet’s membrane. Dutch Opthalmic Society, Groningen, Netherlands, March 2002.
• van Nouhuijs H, van den Berg W, Melles GRJ: Trypan blue stained visco elastic for ophthalmic surgery. Dutch Opthalmic Society, Groningen, Netherlands, March 2002.
• Zijlstra HM, Tan SI, Melles GRJ: On line oogheelkundige verwijzen via www.EyeCT.nl. Dutch Opthalmic Society, Groningen, Netherlands, March 2002.
• Maldonado-Bas A; Barraquer C, Nordan LT, Melles GRJ, Baikoff GD, Malbran E. Lamellar keratoplasty. Instruction course 3104, ASCRS, Philadelphia, USA, April 2002. (Invitation).
• van Dooren BTH, Pels E, Melles GRJ, Beekhuis WH: Biocompatibility of trypan blue with the corneal endothelium. Sydney, Australia, April 2002.
• Melles GRJ: Endo-keratoplasty. Akademie der Augenärzte Münchens, München, Germany, May 2002 (Invitation).
• Melles GRJ: New techniques in keratoplasty and endothelium transplant. VII Congreso de la Sociedad Mediterránea de Oftalmología, Alicante, Spain, May 2002. (Invitation).
• Geerling G, Melles GRJ, Krumeich J, Duncker G: Moderne lamellierende Keratoplastik-Techniken, DOC 2002, Nürnberg, Germany, June 2002. (Invitation).
• Melles GRJ: Lamellar keratoplasty. Argentine Society of Ophthalmology, Buenos Aires, Argentina, July 2002. (Invitation).
• Melles GRJ: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. Instruction course 486, AAO, Orlando, USA, October 2002.
• Melles GRJ: Lamellar keratoplasty. Instruction course 498,  AAO, Orlando, USA, October 2002.
• Aguilera F, Melles GRJ, Mohr A, et al. Trypan blue staining in vitreo-retinal surgery. AAO, Instruction course 294, Orlando, USA, October 2002.
• Aguilera F, Melles GRJ. Trypan blue 0.2% in vitreoretinal surgery. AAO, Video 29, Orlando, USA, October 2002.
• Melles GRJ. Lamellar keratoplasty. International Symposium in Corneal, External Disease and Eye Banking. Sorocoba / Sao Paulo, Brasil, November 2002.

2001
• Melles GRJ: In memoriam: penetrating keratoplasty. Bowman meeting, Liverpool, UK, March 2001. (Invitation).
• Melles GRJ, Nieuwendaal CP: Sutureless, posterior lamellar keratoplasty through a self-sealing 5 mm sclero-corneal tunnel incision. Dutch Opthalmic Society, Maastricht, Netherlands, April 2001.
• Melles GRJ, Nieuwendaal C: Lamellar keratoplasty. Alicante, Spain, April 2001. (Invitation).
• Melles GRJ, Terry MA, Niewendaal CP, Eggink FAGJ: Endothelial Replacement Without Corneal Sutures: Posterior Lamellar Keratoplasty. Instruction course 2101, ASCRS, San Diego, USA, April 2001. • Melles GRJ: Posterior lamellar keratoplasty. Ophthalmic Society of Northern Spain, Pamplona, Spain, May 2001. (Invitation).
• Alio JL, Melles GRJ: Lamellar keratoplasty. SOE, Istanbul, Turkey, June 2001. (Invitation).
• Melles GRJ: Lamellar keratoplasty. Oxford Congress, Oxford, UK, July 2001. (Invitation).
• Melles GRJ: Lamellar keratoplasty. Annual Congress of Ophthalmology, Buenos Aires, Argentina, August 2001. (Invitation).
• Melles GRJ: Lamellar keratoplasty. Turkish Ophthalmology Society, Izmir, Turkey, September 2001. (Invitation).
• Mohr A, Melles GRJ: In-vivo staining of epiretinal membranes and the internal limiting membrane with trypan blue. DOG, Berlin, Germany, September 2001.
• Melles GRJ: Lamellar keratoplasty. Combined meeting of Kerato-Mileusis Study Group VIIIth International Congres / African Society of Refractive Surgery Ist Congress / Tunis Live 2001, Tunis, Tunesia, October 2001. (Invitation).
• Terry M, Melles GRJ. Endothelial replacement without corneal incisions or sutures. Instruction course 100, AAO, New Orleans, USA, October 2001.
• Sarderevic ON, Guell JL, Jackson WB, Maldonado-Bas AR, Melles GRJ, Soong HK, Stark WJ, Tseng SCG, Van Meter WS. Current concepts and new techniques in corneal transplantation. Instruction course 531, AAO, New Orleans, USA, October 2001.
• Aguilera F, Melles GRJ: Trypan blue plus PFCL, the ideal cocktail for removal of a thin ERM and ILM in vitrectomy fro retinal detachment. The Vitreous Society 19th Annual Meeting, Puerto Rico, November 2001.
• Aguilera F, Melles GRJ: Trypan blue 0.3% in vitreoretinal surgery. Third Annual Film Festival. The Vitreous Society 19th Annual Meeting, Puerto Rico, November 2001.

2000
• Melles GRJ: In memoriam: de penetrerende keratoplastiek. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 2000.
• van Overdam K, Melles GRJ: Amnionmembraan transplantatie. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 2000.
• van Overdam K, Melles GRJ, van Meurs J: Trypaan blauw kleuring van epiretinale membranen in-vitro. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 2000.
• van Overdam, B.T.H. van Dooren, G.R.J. Melles: One year follow-up after trypan blue capsule staining in mature cataract surgery. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 2000.
• Melles GRJ, Lander F, van Dooren BTH, Beekhuis WH: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. ASCRS, Boston, USA, May 2000.
• Melles GRJ, Geerards AJM, Remeijer L, Beekhuis WH: Posterior lamellar keratoplasty through a sclerocorneal pocket incision. Wet Lab, ASCRS, Boston, USA, May 2000.
• Melles GRJ, Geerards AJM, Remeijer L, Beekhuis WH: A quick surgical technique for deep anterior lamellar keratoplasty by visco-dissection of Descemet’s membrane. ASCRS, Boston, USA, May 2000.
• Melles GRJ, Lander F, van Dooren BTH, Beekhuis WH: Surgical techniques for deep anterior lamellar keratoplasty, and deep posterior lamellar keratoplasty. Instruction course 1404, ASCRS, Boston, USA, May 2000.
• Melles GRJ, van Overdam K, Vainer O, Ruiz-Perez A, Pandey SK, Werner L, Mohr A: Intraoperative dyes for cataract, corneal, refractive, glaucoma, and retinal surgery. Instruction course 2310, ASCRS, Boston, USA, May 2000.
• Asis Vainer O, Waard de PWT, Escobar-Gomez M, Horiguchi M, Melles GRJ, Pandey SK, Riuz A, Soler F. Capsule dye staining techniques to facilitate mature cataract surgery. Instruction course 4307, ASCRS, Boston, USA, May 2000.
• Melles GRJ, Geerards AJM, Remeijer L, Beekhuis WH: A quick surgical technique for deep anterior lamellar keratoplasty by visco-dissection of Descemet’s membrane. ESCRS, Brussels, Belgium, September 2000.
• van Overdam, B.T.H. van Dooren, G.R.J. Melles: One year follow-up after trypan blue capsule staining in mature cataract surgery. ESCRS, Brussels, Belgium, September 2000.
• Melles GRJ, Lander F, van Dooren BTH, Beekhuis WH: New surgical techniques for deep anterior lamellar keratoplasty, and deep posterior lamellar keratoplasty. Instruction course 22, ESCRS, Brussels, Belgium, September 2000.
• Melles GRJ, Vainer O, Horiguchi M, Soler F, Waard de PWT, Ruiz-Perez A, Pandey SK, Escobar-Gopmes M, van Overdam K: Intraoperative dyes for cataract, corneal, refractive, glaucoma, and retinal surgery. Instruction course 2, ESCRS, Brussels, Belgium, September 2000.
• Terry M, Melles GRJ. Endothelial replacement without corneal incisions or sutures. Instruction course 495, AAO, Dallas, USA, October 2000.
• Sarderevic ON, Guell JL, Jackson WB, Maldonado-Bas AR, Melles GRJ, Soong HK, Stark WJ, Tseng SCG, Van Meter WS. Current concepts and new techniques in corneal transplantation. Instruction course 252, AAO, Dallas, USA, October 2000.

1999
• Melles GRJ, van Dooren BTH, Lander F, Remeijer L, Beekhuis WH, Binder PS: Preliminary clinical results of the Rotterdam technique for posterior lamellar keratoplasty. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• van Overdam KA, Remeijer L, Melles GRJ, Beekhuis WH: Amniotic membrane transplantation in the Rotterdam Eye Hospital. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• de Waard PTW, Melles GRJ, Poort-van Nouhuys HM, Pameijer JH, Beekhuis WH: Trypan blue staining techniques to facilitate phaco-emulsification surgery. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• van Dooren BTH, Kingma J, Pels E, Melles GRJ, Beekhuis WH: Evaluation of cytotoxicity by trypan blue in-vitro. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• van Rooij J, Melles GRJ, Lander F, Geerards AJM, Remeijer L, Beekhuis WH: Preliminary clinical results of the Rotterdam technique for deep, anterior lamellar keratoplasty. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• Melles GRJ, de Waard PTW, Poort-van Nouhuys HM, Pameijer JH, Beekhuis WH: Trypan blue staining to facilitate the capsulorhexis in cataract surgery. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• Christo C, Remeijer L, Geerards AJM, Melles GRJ, van Rooij J, Beekhuis WH: Suture related complications in keratoplasty. Dutch Ophthalmologic Society, Amsterdam, Netherlands, March 1999.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Preliminary results of posterior lamellar keratoplasty. ASCRS, Seattle, USA, April 1999.
• Posterior lamellar keratoplasty through a sclerocorneal pocket incision. Filmfestival, ASCRS, Seattle, USA, April 1999.
• Melles GRJ, de Waard PWT, van Nouhuys HM, Pameijer JH, Beekhuis WH: Trypan blue capsule staining to facilitate the capsulorhexis in mature cataract surgery. ASCRS, Seattle, USA, April 1999.
• Melles GRJ, de Waard PWT: Guidelines to prevent complications in mature cataract surgery. Instruction Course 4405. ASCRS, Seattle, USA, April 1999.
• Melles GRJ, Lander F, Geerards AJM, Remeijer L, Beekhuis WH, Binder PS: Preliminary clinical results of a new technique for deep, anterior lamellar keratoplasty. ASCRS, Seattle, USA, April 1999.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. Instruction Course 2410. ASCRS, Seattle, USA, April 1999.
• Melles GRJ. Panelist: Scientific Paper Session - Refractive: Keratoconus. ASCRS, Seattle, USA, April 1999.
• Melles GRJ, van Marle W: Intraocular lens power calculation after refractive surgery. Alcon Course. Gorinchem, Netherlands, April 1999.
• Eggink FAGJ, ARVO Eggink FAGJ, Melles GRJ, Lander F, Geerards AJM, Remeijer L, Beekhuis WH: preliminary results of the rotterdam technique for deep, anterior lamellar keratoplasty. ARVO, Fort Lauderdale, USA, May 1999.
• van Dooren BTH, Melles GRJ, Lander F, Beekhuis WH, Binder PS: Preliminary clinical results of posterior lamellar keratoplasty. ARVO, Fort Lauderdale, USA, May 1999.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. ESCRS, Vienna, Austria, September 1999.
• de Waard PWT, Melles GRJ, van Nouhuys HM, Pameijer JH, Beekhuis WH: Trypan blue capsule staining to facilitate the capsulorhexis in mature cataract surgery. ESCRS, Vienna, Austria, September 1999.
• de Waard PWT, Melles GRJ: Guidelines to prevent complications in mature cataract surgery. ESCRS, Vienna, Austria, September 1999.
• Melles GRJ, Lander F, Geerards AJM, Remeijer L, Beekhuis WH: Preliminary clinical results of a new technique for deep, anterior lamellar keratoplasty. ESCRS, Vienna, Austria, September 1999.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. Instruction Course 11, ESCRS, Vienna, Austria, September 1999.
• Melles GRJ, de Waard PWT, van Nouhuys HM, Pameijer JH, Beekhuis WH: Trypan blue capsule staining to facilitate the capsulorhexis in mature cataract surgery. Wet lab. ESCRS, Vienna, Austria, September 1999.
• Melles GRJ, Lander F, van Dooren BTH, Pels E, Beekhuis WH: Posterior lamellar keratoplasty through a sclero-corneal pocket incision. AAO, Orlando, USA, October 1999.
• Melles GRJ: Guidelines to prevent complications in mature cataract surgery. Belgian Ophthalmology Society. Brussels, Belgium, November 1999.

1998
• Melles GRJ, Rietveld FJR, Beekhuis WH, Binder PS: A new surgical technique for deep, anterior lamellar keratoplasty. Dutch Ophthalmologic Society, Maastricht, Netherlands, March 1998.
• Melles GRJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: Transplantation of Descemet’s membrane carrying viable endothelium through a small scleral incision. ASCRS, San Diego, USA, April 1998.
• Melles GRJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: Transplantation of Descemet’s membrane carrying viable endothelium through a small scleral incision. ARVO, Fort Lauderdale, USA, May 1998.
• Beekhuis WH, Melles GRJ, Lander F, ten Hoope GW, Rietveld FJR, Remeijer L, Binder PS: A new surgical technique for deep, anterior lamellar keratoplasty. ARVO, Fort Lauderdale, USA, May 1998.
• Melles GRJ, Lander F, Rietveld FJR, Beekhuis WH, Remeijer L, Binder PS: A new surgical technique for deep, anterior lamellar keratoplasty. ESCRS, Nice, France, September 1998.
• Melles GRJ, de Waard PWT, Pameijer JH, Beekhuis WH: Trypan blue capsular staining to visualize capsulorhexis in mature cataract surgery. ESCRS, Nice, France, September 1998.
• Melles GRJ, de Waard PWT, Pameijer JH, Beekhuis WH: Trypan blue capsular staining to visualize capsulorhexis in mature cataract surgery. Netherlands Intraocular Implant Club, Arnhem, USA, October 1998.
• Melles GRJ, Beekhuis WH: Posterior lamellar keratoplasty. Hollandse Oogartsen Avond, Rotterdam, Netherlands, December 1998.

1997
• Melles GRJ, Eggink FAGJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: A potential technique for posterior corneal transplantation. Dutch Ophthalmologic Society, Groningen, Netherlands, March 1997.
• Eggink FAGJ, Melles GRJ, Rietveld FJR, Eggink CA, Beekhuis WH, Binder PS: Donor relaxing trephination before keratoplasty does not reduce postoperative astigmatism in a cat model. Dutch Ophthalmologic Society, Groningen, Netherlands, March 1997.
• Melles GRJ, Caroline PJ, de Sera JP, Eggink CA, Cruysberg JRM, Binder PS: Bilateral, anterior stromal ring opacity of the cornea. Dutch Ophthalmologic Society, Groningen, Netherlands, March 1997.
• Pameyer JH, Melles GRJ: Why does your phaco fail? Dutch Ophthalmologic Society, Groningen, Netherlands, March 1997.
• Eggink FAGJ, Melles GRJ, Rietveld FJR, Eggink CA, Beekhuis WH, Binder PS: Donor relaxing trephination before keratoplasty does not reduce postoperative astigmatism in a cat model. ARVO, Fort Lauderdale, USA, May 1997.
• Melles GRJ, Eggink FAGJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: A potential technique for posterior corneal transplantation. ARVO, Fort Lauderdale, USA, May 1997.
• Melles GRJ, Eggink FAGJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: A potential technique for posterior corneal transplantation. XXIII European Cornea Conference, Buxton, UK, June 1997.
• Melles GRJ, Eggink FAGJ, Rietveld FJR, Pels E, Beekhuis WH, Binder PS: A potential technique for posterior corneal transplantation. 20th Biennial Cornea Research Conference, Boston, USA, September 1997.

Newsletters

 NIIOS Informiert August 2010 (German) (604 KB)
 NIIOS Newsletter August 2010 (Dutch) (518 KB)
 NIIOS Noticias, Mayo 2010 (Spanish) (617 KB)
 NIIOS Informiert, April 2010 (German) (587 KB)
 NIIOS Newsletter March 2010 (Dutch) (616 KB)
 NIIOS Newsletter March 2010 (English) (611 KB)
 NIIOS Newsletter December 2009 (Dutch) (621 KB)
 NIIOS Newsletter, March 2009 (Dutch)(1.45 MB)
 NIIOS Newsletter, March 2009 (English)(1.17 MB)
 NIIOS Informiert, März 2009 (German)(1.30 MB)
 NIIOS Noticias, Marzo 2009 (Spanish)(1.15 MB)
 NIIOS Newsletter, March 2008 (Dutch) (431 KB)  
 NIIOS Newsletter March 2007 (Dutch) (272 KB)
 NIIOS Newsletter, October 2007 (Dutch) (398 KB)
 NIIOS Newsletter, October 2007 (English) (953 KB) 
 NIIOS Newsletter, March 2006 (Dutch) (355 KB)
 NIIOS Newsletter, March 2005 (Dutch) (419 KB)
 NIIOS Newsletter, October 2005 (Dutch) (341 KB) 
 NIIOS Newsletter, March 2004 (Dutch) (117 KB)
 NIIOS Newsletter, November 2004 (Dutch) (163 KB) 
 NIIOS Newsletter, April 2003 (Dutch) (253 KB) 
 

Annual reports

 Annual report 2005 (1.3 MB)  

Public press releases

2010
• Boyle EL, Mullin DW. DMEK shows good visual results at 6 months. Ocular Surgery News, September 2010.
• Melles GRJ. Donorweefsel beter gewogen. Medisch Contact, June 2010.
• Budde B. De mogelijkheden zijn zoveel groter dan we aankunnen. VOZ Magazine, March 2010.
• Melles GRJ. Alle DSAEK's vervangen met DMEK? Moeten alle DSAEK transplantaten nu vervangen worden door DMEK transplantaten? Congreskrant 204e Jaarvergadering NOG, March 2010.
• van der Wees J. Endotheelceloverleving drie jaar na DMEK-operatie. Getransplanteerde cellen houden zich goed. Congreskrant 204e Jaarvergadering NOG, March 2010.
• Massimo B, Melles GRJ, Nuijts R. Endothelial keratoplasty challenges the primacy of full-thickness transplantation. Ocular Surgery News, April 2010.

2009
• Priegelen op de vierkante millimeter. Scope, December 2009
• Dalton M. Cor

2008
• Helderheid in hoornvliestransplantatie. Medisch Contact 2008;63:184-186.
• Corneal graft technique aims to replace only Descemet's membrane. Ocular Surgery News (Europe/Asia-Pacific Edition), January 2008.
• Hitchin L. Hippocratech Surgical’s SurgiCube Operation Unit. IMTJ Online, Technological Advantage 2008;Chapter 4.
• Organ-cultured keratoplasty technique shows potential for treating endothelial disorders. Ocular Surgery News, February 2008.
• Keratoplasty technique based on organ-cultured Descemet's transplantation shows promise. Ocular Surgery News, February 2008.
• SurgiCube Hippocratech. Ophthalmology Times Europe, March 2008.
• Descemet membrane endothelial keratoplasty: Binnen 1-3 maanden 90% > 0.5 visus en 50% > 0.8 visus. Congreskrant 202e Jaarvergadering NOG, April 2008.
• Effectief praktijkmanagement met de SurgiCube. Ingreep buiten de operatiekamer bespaart kostbare tijd. Congreskrant 202e Jaarvergadering NOG, April 2008.
• Dalton M. PKP to DMEK: An update on corneal transplantation techniques. Eyeworld, May 2008.
• DMEK graft failure not primarily from tissue manipulation. Ocular Surgery News, in press.

2007
• SurgiCube: de innovatieve operatieruimte. NOG Congreskrant, March 2007.
• Hoornvliestransplantatie nadert perfectie! Descemet membraan transplantatie: binnen 1 week 100% visus. NOG Congreskrant, March 2007.
• Calmthout M. Rotterdamse oogchirurgen matten door tot de KNAW. Volkskrant, April 7 2007.
• Deep inside keratoplasty. Eyeworld, May 2007.
• DMEK results favourable. Eurotimes Today, September 2007.
• Henahan S. DMEK results favourable in Stockholm. Eurotimes, November 2007.